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The coverslips were then washed with PBS and the cells were fixed with 4% PFA for 15 min, washed with 0.05% Tween 20 (Promega, WI, USA) diluted in PBS (PBST), permeabilized with 0.2% Triton X-100 (Promega, WI, USA) for 10 min, and blocked with 3% BSA in PBST for 3 h

Heres the Navys summaryif you want to read the full reportsand Admiral Greenerts endorsementgo here: The investigation determined that the likely cause of the fire was unauthorized smoking that ignited flammable liquids and other combustible material improperly stored in an adjacent space

Using excessively strong pouches can cause nicotine overdose and other side effects